Analysis Note

ControlRat brain tissue lysate

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in HeLa cells (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network.

Immunogen

Recombinant full length human Dynamin-1 expressed in transfected Sf9 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate.

Specificity

This antibody detects both Dynamin-1 and Dynamin-2. (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed.
Dynamin 1 has a calculated MW of 97 kDa Dynamin 2 has a calcluated MW of 98 kDa